Human SIRT1 (Sirtuin 1) CLIA Kit

Specifications

Catalog number

E-CL-H0992-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SIRT1

UniProt

Q96EB6

Cross Reactivity

This kit recognizes Human SIRT1 in samples.No significant cross-reactivity or interference between Human SIRT1 and analogues was observed

Target

SIR2L1; NAD-dependent deacetylase sirtuin-1

Type

Sandwich-ELISA

Applications

CLIA

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Tags and Cell Markers

Assay Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the RLU value of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,6 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human SIRT1 concentrations in Tissue homogenates, cell lysates and other biological fluids.