Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit — image 1 of 3

Product details

Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

Catalog number E-BC-F032-01

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Price for E-BC-F032-01

Size / count 48 T (32 samples)

Specifications

Catalog number: E-BC-F032-01
Size / count: 48 T (32 samples)
Dry ice: No
Hazardous: No
Discontinued: No
Type: Fluorometric method
Field of Research: Glycolysis and Lipid Metabolism
Assay Principle: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE) . Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Assay Performance Time: 20 min
Sample Type: Serum; plasma; animal tissue; cells
Detection Range: 0.12-30 μmol/L
Sensitivity: 0.12 μmol/L
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://www.mdpi.com/1908036
Inter CV: 7.3%
Intra CV: 1.7%
Detection Instrument: Fluorescence Microplate Reader

Frequently Asked Questions

What is the catalog number for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit?

The catalog number for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is E-BC-F032.

What sizes are available for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit?

Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is available in the following size(s): 48 T (32 samples), 96 T (80 samples).

How many pack sizes does Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit come in?

Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is available in 2 different pack sizes.

Is Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit in stock?

Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit?

Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is manufactured by Elabscience.

What is the estimated delivery time for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit?

Estimated delivery time for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit?

The safety data sheet for Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit is available here: https://789.bio/eb/S04mHO.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.