Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F038-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

ALT

Type

Fluorometric method

Field of Research

Liver and Renal Function

Assay Principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Assay Performance Time

75 min

Sample Type

Serum; plasma; animal tissue

Detection Range

0.01-0.83 U/L

Sensitivity

0.01 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S1742706120307509

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acsami.9b19518

Citation 04

https://www.sciencedirect.com/science/article/pii/S0278691521001575

Citation 05

https://www.mdpi.com/2039948

Citation 06

https://www.nature.com/articles/s41598-022-21816-x

Inter CV

9.8%

Intra CV

2.3%

Detection Instrument

Fluorescence Microplate Reader