Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F039-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

FFA; NEFA

Type

Fluorometric method

Field of Research

Metabolic Diseases; Glycolysis and Lipid Metabolism

Assay Principle

Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Assay Performance Time

70 min

Sample Type

Serum; plasma; animal tissue; cells

Detection Range

1.20-100 μmol/L

Sensitivity

1.20 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.nature.com/articles/s41467-022-35481-1

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

Citation 03

https://link.springer.com/article/10.1186/s13098-022-00921-x

Citation 04

https://www.sciencedirect.com/science/article/pii/S0378874122009011

Citation 05

https://www.sciencedirect.com/science/article/pii/S0955286319301330

Citation 06

https://link.springer.com/article/10.1007/s11356-021-14871-w

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/01480545.2020.1784190

Inter CV

5.1%

Intra CV

4.1%

Detection Instrument

Fluorescence Microplate Reader