Dipeptidyl Peptidase IV (DPP4) Activity Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F053

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

DPP4

Type

Fluorometric method

Field of Research

Amino Acids and Proteins

Assay Principle

Dipeptidyl Peptidase IV (DPP4), also known as CD26, is a kind of serine protease, can decompose the second N-terminal proline or alanine residue of the peptide chain. In organisms, dipeptidyl peptidase can rapidly decompose incretin, which can stabilize insulin level and promote the reduction of blood glucose level in organisms. The detection principle of this kit is that DPP4 can decompose the substrate and release the fluorescent substance AMC, and the activity of this enzyme can be inhibited by adding DPP4 inhibitor. The activity of DPP4 could be calculated by the difference of fluorescence value before and after inhibition.

Assay Performance Time

70 min

Sample Type

Serum; plasma; tissue; cell

Detection Range

0.92-3.77 U/L

Sensitivity

0.92 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

5.7%

Intra CV

0.6%

Detection Instrument

Fluorescence microplate reader