D-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K002-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

D-LA

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A

Assay Performance Time

20 min

Sample Type

Serum; plasma; animal tissue; saliva sample

Detection Range

0.06-8.0 mmol/L

Sensitivity

0.06 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-022-01390-0

Citation 02

https://www.tandfonline.com/doi/abs/10.2147/IJN.S394544

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13245

Citation 04

https://www.mdpi.com/1999-4923/14/9/1759

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8716831/

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977854/

Citation 08

https://www.mdpi.com/1400748

Citation 09

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.2c04017

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202100253

Citation 11

https://link.springer.com/article/10.1007/s12094-022-03008-1

Citation 12

https://rep.bioscientifica.com/view/journals/rep/160/6/REP-20-0381.xml

Inter CV

7.7%

Intra CV

3.8%

Detection Instrument

Microplate reader