Free Cholesterol (FC) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K004-M-01

Size / count

48 T (44 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

FC

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.

Assay Performance Time

20 min

Sample Type

Serum; plasma; tissue

Detection Range

0.07-24 mmol/L

Sensitivity

0.07 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0002944022002425

Citation 02

https://www.sciencedirect.com/science/article/pii/S0377840121001942

Citation 03

https://www.tandfonline.com/doi/abs/10.2147/VHRM.S396295

Inter CV

5.2%

Intra CV

1.9%

Detection Instrument

Microplate reader