Glucose-6-phosphate (G6P) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K011-M

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

G6P

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism; Disulfidptosis

Assay Principle

Glucose-6-phosphate (G6P) is a molecule generated by phosphorylation of hydroxyl groups on the sixth carbon of glucose under the catalysis of hexokinase. It is a common small molecule of sugar metabolism in cells and participates in biochemical pathways such as glycolysis and pentose phosphate pathway. In the first reaction of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis: In the pentose phosphate pathway, glucose-6-phosphate is the first substrate, and this process is also the main way to generate NADPH. In addition to these two metabolic pathways, glucose-6-phosphate can also be converted into glycogen or starch and stored.

Assay Performance Time

35 min

Sample Type

Serum; plasma; animal tissue

Detection Range

5.6-500 μmol/L

Sensitivity

5.6 μmol /L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

4.3%

Intra CV

2.1%

Detection Instrument

Microplate reader