Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

Specifications

Catalog number

E-BC-K057-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

ALB

Type

Colorimetric method

Field of Research

Liver and Renal Function; Amino Acids and Proteins

Assay Principle

Bromocresol green (BCG) is widely used as protein staining agent. BCG can combine the albumin in pH 4.0~4.2 to form an albumin-BCG complex. And the color changed from yellow to green. The depth of color is proportional to the concentration of albumin. The content of albumin in serum can be calculated indirectly by measuring the OD value at 630 nm.

Assay Performance Time

20 min

Sample Type

Serum; plasma

Detection Range

0.08-15 g/L

Sensitivity

0.08 g/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748081/

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525417/

Citation 04

https://www.mdpi.com/1433254

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882294/

Citation 06

https://www.jstage.jst.go.jp/article/jos/71/9/71_ess22033/_article/-char/ja/

Citation 07

https://journals.sagepub.com/doi/abs/10.1177/08968608211014568

Inter CV

4.6%

Intra CV

1.5%

Detection Instrument

Microplate reader