Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K071-M-01

Size / count

48 T (31 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

TIBC

Type

Colorimetric method

Field of Research

Inorganic ions

Assay Principle

The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC) .

Assay Performance Time

20 min

Sample Type

Serum

Detection Range

0.31-50 mg/L

Sensitivity

0.14 mg/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo02226e

Inter CV

2.3%

Intra CV

1.5%

Detection Instrument

Microplate reader