Alkaline Phosphatase (ALP) Activity Assay Kit

Specifications

Catalog number

E-BC-K091-S-01

Size / count

100 Assays (96 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

ALP

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Assay Performance Time

60 min

Sample Type

Serum; plasma; urine; tissue; cells

Detection Range

0.2-55.6 King unit/1

Sensitivity

0.2 King unit/100 mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://advances.sciencemag.org/content/5/8/eaax0064?utm_source=TrendMD&utm_medium=cpc&utm_campaign=TrendMD_1

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acs.est.2c00516

Citation 03

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01646-9

Citation 04

https://www.sciencedirect.com/science/article/pii/S0304389420328673

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.30919

Citation 06

https://www.mdpi.com/2073-4409/10/7/1622

Citation 07

https://www.sciencedirect.com/science/article/pii/S0944711322006055

Citation 08

https://www.frontiersin.org/articles/10.3389/fphar.2021.756924/full

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579055/

Citation 10

https://www.nature.com/articles/s41598-022-21816-x

Citation 11

https://www.sciencedirect.com/science/article/pii/S875632822100377X

Citation 12

https://link.springer.com/article/10.1007/s12598-021-01814-0

Inter CV

5.6%

Intra CV

2.1%

Detection Instrument

Spectrophotometer