Glutathione Peroxidase (GSH-Px) Activity Assay Kit — image 1 of 2

Product details

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Catalog number E-BC-K096-S-01

Choose a variant (2 options)

Selecting a variant updates its price, size and availability.

Price for E-BC-K096-S-01

Size / count 50 Assays (24 samples)

Specifications

Catalog number: E-BC-K096-S-01
Size / count: 50 Assays (24 samples)
Dry ice: No
Hazardous: No
Discontinued: No
Target: GSH-PX
Type: Colorimetric method
Field of Research: Oxidative Stress; Plant Physiology Series; Ferroptosis
Assay Principle: Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG) . The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Assay Performance Time: 70 min
Sample Type: Serum; plasma; cells; cell culture supernatant; tissue
Detection Range: 12.65-387 U
Sensitivity: 12.65 U
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at 2~8°C for 12 months with shading light.
Citation 01: https://www.mdpi.com/article/10.3390/antiox11112264
Citation 02: https://www.mdpi.com/2072-6643/14/15/3179
Citation 03: https://www.sciencedirect.com/science/article/pii/S0753332221008003
Citation 04: https://www.sciencedirect.com/science/article/pii/S0278691522002599
Citation 05: https://www.mdpi.com/2076-3921/9/3/250
Citation 06: https://www.sciencedirect.com/science/article/pii/S0024320521012170
Citation 07: https://www.hindawi.com/journals/omcl/2020/2978340/
Citation 08: https://www.sciencedirect.com/science/article/pii/S1001074222000717
Citation 09: https://www.mdpi.com/2077-0383/7/10/315
Citation 10: https://pubmed.ncbi.nlm.nih.gov/36160397/
Citation 11: https://www.mdpi.com/1332228
Citation 12: https://www.sciencedirect.com/science/article/pii/S0009279720310875
Inter CV: 9.3%
Intra CV: 4.9%
Detection Instrument: Spectrophotometer

Frequently Asked Questions

What is the catalog number for Glutathione Peroxidase (GSH-Px) Activity Assay Kit?

The catalog number for Glutathione Peroxidase (GSH-Px) Activity Assay Kit is E-BC-K096-S.

What sizes are available for Glutathione Peroxidase (GSH-Px) Activity Assay Kit?

Glutathione Peroxidase (GSH-Px) Activity Assay Kit is available in the following size(s): 50 Assays (24 samples), 100 Assays (48 samples).

How many pack sizes does Glutathione Peroxidase (GSH-Px) Activity Assay Kit come in?

Glutathione Peroxidase (GSH-Px) Activity Assay Kit is available in 2 different pack sizes.

Is Glutathione Peroxidase (GSH-Px) Activity Assay Kit in stock?

Glutathione Peroxidase (GSH-Px) Activity Assay Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Glutathione Peroxidase (GSH-Px) Activity Assay Kit?

Glutathione Peroxidase (GSH-Px) Activity Assay Kit is manufactured by Elabscience.

What is the estimated delivery time for Glutathione Peroxidase (GSH-Px) Activity Assay Kit?

Estimated delivery time for Glutathione Peroxidase (GSH-Px) Activity Assay Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Glutathione Peroxidase (GSH-Px) Activity Assay Kit?

The safety data sheet for Glutathione Peroxidase (GSH-Px) Activity Assay Kit is available here: https://789.bio/eb/PWnHW1.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.