Total Glutathione (T-GSH) /Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K097-S-01

Size / count

50 Assays (48 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

T-GSH; GSSG

Type

Colorimetric method

Field of Research

Oxidative stress

Assay Principle

GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Assay Performance Time

180 min

Sample Type

Serum; plasma; animal tissue; red blood cells; cultured cells

Detection Range

0.12-30 μmol/L T-GSH

Sensitivity

0.12 μmol/L T-GSH

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0308814621004301

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03577k

Citation 03

https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15397

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/13880209.2022.2070221

Citation 05

https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00275-w

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.11611

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.2024319

Inter CV

4.7%

Intra CV

0.9%

Detection Instrument

Spectrophotometer