Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Specifications

Catalog number

E-BC-K125-S-01

Size / count

50 Assays (25 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

ChAT

Type

Colorimetric method

Field of Research

Amino Acids and Proteins

Assay Principle

Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

Assay Performance Time

80 min

Sample Type

Animal tissue

Detection Range

1.21-40 U/g fresh we

Sensitivity

1.21 U/g fresh weigh

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.mdpi.com/2076-3921/10/6/835

Citation 02

https://www.mdpi.com/2076-3921/9/7/620

Citation 03

https://link.springer.com/article/10.1007/s11064-022-03684-z

Citation 04

https://www.mdpi.com/2050368

Citation 05

https://link.springer.com/article/10.1007/s13596-022-00653-3

Inter CV

9.2%

Intra CV

5%

Detection Instrument

Spectrophotometer