Mitochondrial Complex III (Coenzyme Q-Cytochrome c Reductase) Activity Assay Kit

Specifications

Catalog number

E-BC-K151-M-01

Size / count

48 T (23 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Colorimetric method

Field of Research

Mitochondria Function; cuproptosis

Assay Principle

As an organelle, mitochondria is the "power factory" in cells and the main site of aerobic respiration of cells. Its function is to convert energy through oxidative phosphorylation to provide energy for cellular activities. The oxidation process is carried out by four respiratory chain membrane protein complexes (complexes I, II, III and IV) on the inner mitochondrial membrane. mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial complex III can be quantified by measure the change OD value at 550 nm.

Assay Performance Time

10 min

Sample Type

Animal tissue

Detection Range

4.45–106.8 U/L

Sensitivity

4.45 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

10%

Intra CV

5%

Detection Instrument

Microplate reader