Total Bile Acid (TBA) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K181-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

TBA

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.

Assay Performance Time

25 min

Sample Type

Serum; animal tissue

Detection Range

1.77-40 μmol/L

Sensitivity

1.36 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.mdpi.com/2098480

Citation 02

https://www.sciencedirect.com/science/article/pii/S2212429222005399

Citation 03

https://www.mdpi.com/1861292

Citation 04

https://www.hindawi.com/journals/ecam/2022/7832540/

Inter CV

3.7%

Intra CV

3.4%

Detection Instrument

Microplate reader