Alanine Aminotransferase (ALT/GPT) Activity Assay Kit — image 1 of 2

Product details

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Catalog number E-BC-K235-S-01

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Price for E-BC-K235-S-01

Size / count 50 Assays (19 samples)

Specifications

Catalog number: E-BC-K235-S-01
Size / count: 50 Assays (19 samples)
Dry ice: No
Hazardous: No
Discontinued: No
Target: ALT; GPT; Glutamic-pyruvic transaminase
Type: Colorimetric method
Field of Research: Liver and Renal Function
Assay Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Assay Performance Time: 100 min
Sample Type: Serum; plasma; cells; animal tissue
Detection Range: 1.26-72.3 IU/L
Sensitivity: 1.26 IU/L
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at 2~8°C for 12 months with shading light.
Citation 01: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009960
Citation 02: https://www.mdpi.com/2073-4409/10/7/1622
Citation 03: https://www.sciencedirect.com/science/article/pii/S0378874122003452
Citation 04: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-022-03536-x
Citation 05: https://peerj.com/articles/13082/
Citation 06: https://www.hindawi.com/journals/jnm/2021/7512957/
Citation 07: https://journals.sagepub.com/doi/abs/10.1177/0748233719893203
Citation 08: https://www.sciencedirect.com/science/article/pii/S2214750017301051
Inter CV: 8.2%
Intra CV: 4.3%
Detection Instrument: Spectrophotometer

Frequently Asked Questions

What is the catalog number for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit?

The catalog number for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is E-BC-K235-S.

What sizes are available for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit?

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is available in the following size(s): 50 Assays (19 samples), 100 Assays (44 samples).

How many pack sizes does Alanine Aminotransferase (ALT/GPT) Activity Assay Kit come in?

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is available in 2 different pack sizes.

Is Alanine Aminotransferase (ALT/GPT) Activity Assay Kit in stock?

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Alanine Aminotransferase (ALT/GPT) Activity Assay Kit?

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is manufactured by Elabscience.

What is the estimated delivery time for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit?

Estimated delivery time for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit?

The safety data sheet for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit is available here: https://789.bio/eb/f5OqPS.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.