Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Specifications

Catalog number

E-BC-K650-M

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

PDH

Type

Colorimetric method

Field of Research

Tricarboxylic Acid (TCA) Cycle

Assay Principle

Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.

Assay Performance Time

15 min

Sample Type

Serum (plasma) ; urine; animal tissue; cell

Detection Range

0.28-168.91 U/L

Sensitivity

0.28 U /L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

10%

Intra CV

2%

Detection Instrument

Microplate reader