Β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K785-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

Β-HB

Type

Colorimetric method

Field of Research

Metabolic Diseases; Glycolysis and Lipid Metabolism

Assay Principle

Β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.

Assay Performance Time

60 min

Sample Type

Serum; plasma; urine; animal tissue

Detection Range

0.01-2.00 mmol/L

Sensitivity

0.01 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Inter CV

4.0%

Intra CV

2.0%

Detection Instrument

Microplate reader