Total Oxidant Status (TOS) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K802-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

TOS

Type

Colorimetric method

Field of Research

Oxidative stress

Assay Principle

Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.

Assay Performance Time

30 min

Sample Type

Cell; tissue; serum; other liquid samples

Detection Range

2.5-100 μmol H2O2 Equiv./L

Sensitivity

2.5 μmol H2O2 Equiv./L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.hindawi.com/journals/omcl/2022/4591134/

Citation 02

https://www.mdpi.com/2079-7737/10/9/820

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/jbt.23083

Citation 04

https://link.springer.com/article/10.1007/s12011-022-03275-7

Inter CV

3.5%

Intra CV

2.3%

Detection Instrument

Microplate reader