NADP+/NADPH Colorimetric Assay Kit (WST-8) — image 1 of 3

Product details

NADP+/NADPH Colorimetric Assay Kit (WST-8)

Catalog number E-BC-K803-M

Price for E-BC-K803-M

Size / count 96 T (80 samples)

Specifications

Catalog number: E-BC-K803-M
Size / count: 96 T (80 samples)
Dry ice: No
Hazardous: No
Discontinued: No
Target: NADP; NADPH
Type: Colorimetric method
Field of Research: Oxidative Stress; Metabolic Diseases; Glycolysis and Lipid Metabolism; Cuproptosis; Disulfidptosis
Assay Principle: Detect total content of NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm. Detect NADPH: After treating sample, heat at 60°C water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm. Detect NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH.
Assay Performance Time: 70 min
Sample Type: Animal tissu; cells
Detection Range: 0.02-5.0 μmol/L
Sensitivity: 0.02 μmol/L
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://www.mdpi.com/2076-3921/11/9/1692
Citation 02: https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.28222
Citation 03: https://onlinelibrary.wiley.com/doi/abs/10.1111/jdi.13690
Citation 04: https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.23740
Inter CV: 5.5%
Intra CV: 2.1%
Detection Instrument: Microplate reader

Frequently Asked Questions

What is the catalog number for NADP+/NADPH Colorimetric Assay Kit (WST-8)?

The catalog number for NADP+/NADPH Colorimetric Assay Kit (WST-8) is E-BC-K803-M.

What sizes are available for NADP+/NADPH Colorimetric Assay Kit (WST-8)?

NADP+/NADPH Colorimetric Assay Kit (WST-8) is available in the following size(s): 96 T (80 samples).

Is NADP+/NADPH Colorimetric Assay Kit (WST-8) in stock?

NADP+/NADPH Colorimetric Assay Kit (WST-8) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures NADP+/NADPH Colorimetric Assay Kit (WST-8)?

NADP+/NADPH Colorimetric Assay Kit (WST-8) is manufactured by Elabscience.

What is the estimated delivery time for NADP+/NADPH Colorimetric Assay Kit (WST-8)?

Estimated delivery time for NADP+/NADPH Colorimetric Assay Kit (WST-8) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for NADP+/NADPH Colorimetric Assay Kit (WST-8)?

The safety data sheet for NADP+/NADPH Colorimetric Assay Kit (WST-8) is available here: https://789.bio/eb/iLebvH.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.