NAD+/NADH Colorimetric Assay Kit (WST-8)

Specifications

Catalog number

E-BC-K804-M

Size / count

96 T (80 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

+

Type

Colorimetric method

Field of Research

Oxidative stress; Metabolic Diseases; Glycolysis and Lipid Metabolism

Assay Principle

Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.

Assay Performance Time

70 min

Sample Type

Animal tissue; cell

Detection Range

0.02-5.0 μmol/L

Sensitivity

0.02 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

9.1%

Intra CV

1.8%

Detection Instrument

Microplate reader