Oxaloacetate Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K901-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Colorimetric method

Field of Research

Tricarboxylic Acid (TCA) Cycle

Assay Principle

Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.

Assay Performance Time

15 min

Sample Type

Animal tissue

Detection Range

0.017-1 mmol/L

Sensitivity

0.017 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

2.3%

Intra CV

2.1%

Detection Instrument

Microplate reader