Malic Acid Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K905-M

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Colorimetric method

Field of Research

Tricarboxylic Acid (TCA) Cycle

Assay Principle

L-malic acid, also known as 2-hydroxysuccinic acid, is widely present in living organisms. It has two stereoisomers and is one of the members of the tricarboxylic acid cycle, an important metabolic cycle in living organisms. The detection principle of this kit is that the enzyme catalyzes malic acid to produce oxaloacetic acid. At the same time, under the action of electronic coupler, the chromogenic agent is reduced to produce orange yellow product, which has the maximum absorption peak at about 450 nm.

Sample Type

Serum (plasma) ; tissue; cell

Detection Range

0.003-0.5 mmol/L

Sensitivity

0.003 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

4.6-5.9%

Intra CV

2.6-3.6%

Detection Instrument

Microplate reader