PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1]

Specifications

Catalog number

E-AB-F0995G-01

Size / count

50 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Background

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF) -transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Abbreviation

F4/80

Gene ID

13733

UniProt

Q61549

Host

Rat

Reactivity

Mouse

Target

Gpf480; Adgre1; Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; Emr1

Clonality

Monoclonal

Clone

CI:A3-1

Conjugation

PE/Cyanine 5

Applications

FCM

Field of Research

Cell Biology; Immunology; Innate Immunity; Neuroinflammation; Neuroscience

Concentration

5 μL/Test

Buffer

Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/ctm2.1742

Recommended Usage

The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) .

Isotype

Rat IgG2b, κ