AF/LE Purified Anti-Human CD3 Antibody[OKT-3]

Specifications

Catalog number

E-AB-F10010-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Abbreviation

CD3

Gene ID

916

UniProt

P07766

Host

Mouse

Reactivity

Human

Immunogen

Recombinant Human CD3 protein

Target

CD3E; CD3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E

Clonality

Monoclonal

Clone

OKT3

Conjugation

None (AF/LE)

Applications

FCM

Field of Research

Immunology

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Citation 01

https://isevjournals.onlinelibrary.wiley.com/doi/abs/10.1002/jev2.12490

Citation 02

https://www.liebertpub.com/doi/abs/10.1089/jir.2023.0043

Recommended Usage

6

Isotype

Mouse IgG2a, κ