Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]

Specifications

Catalog number

E-AB-F1018A-01

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) . CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Abbreviation

CD54

Gene ID

15894

UniProt

P13597

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant Mouse CD54 protein

Target

CD54; Icam-1; Icam1; Intercellular adhesion molecule 1; MALA-2; MyD10

Clonality

Monoclonal

Clone

YN1/1.7.4

Conjugation

Unconjugated

Applications

FCM

Field of Research

Cell Adhesion; Cell Biology; Costimulatory Molecules; Immunology; Innate Immunity; Neuroscience; Neuroscience Cell Markers; Stem Cells

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Recommended Usage

6

Isotype

Rat IgG2b, κ