AF/LE Purified Anti-Mouse CD22 Antibody[Cy34.1]

Specifications

Catalog number

E-AB-F10210-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL) . CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.

Abbreviation

CD22

Gene ID

12483

UniProt

P35329

Host

Mouse

Reactivity

Mouse

Target

Siglec2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; Cd22; Lyb-8; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14

Clonality

Monoclonal

Clone

Cy34.1

Conjugation

None (AF/LE)

Applications

FCM

Field of Research

Immunology

Concentration

≥ 1 mg/mL

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

6

Isotype

Mouse IgG1, κ