Purified Anti-Mouse CD11a Antibody[FD441.8]

Specifications

Catalog number

E-AB-F1033A-01

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

Abbreviation

CD11a

Gene ID

16408

UniProt

P24063

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant Mouse CD11a protein

Target

CD11 antigen-like family member A; CD11a; Integrin alpha-L; Itgal; LFA-1A; Ly-15; Lymphocyte antigen 15

Clonality

Monoclonal

Clone

FD441.8

Conjugation

Unconjugated

Applications

FCM

Field of Research

Cell Adhesion; Cell Biology; Costimulatory Molecules; Immunology; Innate Immunity; Neuroinflammation; Neuroscience

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

PBS, pH 7.2. Contains 0.05% proclin 300.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Recommended Usage

6

Isotype

Rat IgG2b, κ