Purified Anti-Mouse CD162 Antibody[4RA10]

Specifications

Catalog number

E-AB-F1034A-01

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Abbreviation

CD162

UniProt

Q62170

Host

Rat

Reactivity

Mouse

Target

CD162; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; Selplg

Clonality

Monoclonal

Clone

4RA10

Conjugation

Unconjugated

Applications

FCM; Block

Field of Research

Cell Adhesion; Immunology

Concentration

≥ 1 mg/mL

Buffer

Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Recommended Usage

6

Isotype

Rat IgG1, κ