PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10]

Specifications

Catalog number

E-AB-F1034I-01

Size / count

50 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Background

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Abbreviation

CD162

Gene ID

20345

UniProt

Q62170

Host

Rat

Reactivity

Mouse

Target

CD162; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; Selplg; PSGL1

Clonality

Monoclonal

Clone

4RA10

Conjugation

PE/Cyanine 5.5

Applications

FCM

Field of Research

Cell Adhesion; Immunology

Concentration

5 μL/Test

Buffer

Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Recommended Usage

The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) .

Isotype

Rat IgG1, κ