AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7]

Specifications

Catalog number

E-AB-F11040-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α (CD8a) /β (CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Abbreviation

CD8a

Gene ID

12525

UniProt

P01731

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant Mouse CD8a protein

Target

CD8A; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Clonality

Monoclonal

Clone

53-6.7

Conjugation

None (AF/LE)

Applications

FCM

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Citation 01

https://pubs.rsc.org/en/content/articlelanding/2024/tb/d4tb01917b

Recommended Usage

6

Isotype

Rat IgG2a, κ