AF/LE Purified Anti-Human CD8a Antibody[OKT-8]

Specifications

Catalog number

E-AB-F11100-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers) . CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Abbreviation

CD8

Gene ID

925

UniProt

P01732

Host

Mouse

Reactivity

Human

Immunogen

Recombinant Human CD8 protein

Target

CD8A; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Clonality

Monoclonal

Clone

OKT-8

Conjugation

None (AF/LE)

Applications

FCM

Field of Research

Immunology

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

6

Isotype

Mouse IgG2a, κ