AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]

Specifications

Catalog number

E-AB-F11320-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Abbreviation

CD274/PD-L1

Gene ID

60533

UniProt

Q9EP73

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant Mouse CD274 protein

Target

B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; B7 homolog 1; B7-H; B7-H1; Programmed cell death ligand 1

Clonality

Monoclonal

Clone

10F.9G2

Conjugation

None (AF/LE)

Applications

FCM

Field of Research

Cancer Biomarkers; Costimulatory Molecules; Immunology

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

6

Isotype

Rat IgG2b, κ