Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3]

Specifications

Catalog number

E-AB-F1133A-01

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1) . This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.

Abbreviation

PD-L1

Gene ID

29126

UniProt

Q9NZQ7

Host

Mouse

Reactivity

Human

Immunogen

Recombinant Human PD-L1 protein

Target

B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; B7 homolog 1; B7-H; B7-H1; Programmed cell death ligand 1

Clonality

Monoclonal

Clone

29E.2A3

Conjugation

Unconjugated

Applications

FCM

Field of Research

Cancer Biomarkers; Costimulatory Molecules; Immunology

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Recommended Usage

6

Isotype

Mouse IgG2b, κ