PE/Cyanine5 Anti-Human CD45 Antibody[HI30]

Specifications

Catalog number

E-AB-F1137G-01

Size / count

20 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Abbreviation

CD45

Gene ID

5788

UniProt

P08575

Host

Mouse

Reactivity

Human

Target

CD45; L-CA; Ly-5; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200

Clonality

Monoclonal

Clone

HI30

Conjugation

PE/Cyanine 5

Applications

FCM

Field of Research

Cell Biology; Immunology; Inhibitory Molecules; Innate Immunity; Neuroscience; Neuroscience Cell Markers; Stem Cells

Concentration

5 μL/Test

Buffer

Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Recommended Usage

The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood) .

Isotype

Mouse IgG1, κ