AF/LE Purified Anti-Human IL-4 Antibody[MP4-25D2]

Specifications

Catalog number

E-AB-F12030-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Abbreviation

IL-4

Gene ID

3565

UniProt

P05112

Host

Rat

Reactivity

Human

Target

B-cell IgG differentiation factor; B-cell growth factor 1; BSF-1; IGG1 induction factor; IL-4; Interleukin-4

Clonality

Monoclonal

Clone

MP4-25D2

Conjugation

None (AF/LE)

Applications

ICFCM; Neut

Field of Research

Immunology

Concentration

≥ 1 mg/mL

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Purified MP4-25D2 has been tested by blocking fluorochrome conjugated MP4-25D2 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 µg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype

Rat IgG1, κ