AF/LE Purified Anti-Mouse IL-4 Antibody[11B11]

Specifications

Catalog number

E-AB-F12040-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.

Abbreviation

IL-4

Gene ID

16189

UniProt

P07750

Host

Rat

Reactivity

Mouse

Target

B-cell IgG differentiation factor; B-cell growth factor 1; BSF-1; IGG1 induction factor; IL-4; Interleukin-4

Clonality

Monoclonal

Clone

11B11

Conjugation

None (AF/LE)

Applications

ICFCM; Neut; Stim

Field of Research

Immunology

Concentration

≥ 1 mg/mL

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISPOT applications, a concentration range of 2-6 μg/ml is recommended. For ELISA capture applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype

Rat IgG1, κ