AF/LE Purified Anti-Mouse IL-6 Antibody[MP5-20F3]

Specifications

Catalog number

E-AB-F12070-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Abbreviation

IL-6

UniProt

P08505

Host

Rat

Reactivity

Mouse

Target

B-cell hybridoma growth factor; IL-6; Interleukin HP-1; Interleukin-6

Clonality

Monoclonal

Clone

MP5-20F3

Conjugation

None (AF/LE)

Applications

ICFCM; Neut

Field of Research

Immunology; Innate Immunity

Concentration

≥ 1 mg/mL

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.5 – 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL are recommended for each ELISA plate. For ELISPOT applications, a concentration range of 4.0 - 8.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype

Rat IgG1, κ