HIF1AN Polyclonal Antibody

Specifications

Catalog number

E-AB-13556-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.

Abbreviation

HIF1AN

UniProt

Q9NWT6

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Synthetic peptide of human HIF1AN

Target

DKFZp762F1811; Factor inhibiting HIF-1; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; FLJ20615; FLJ22027 ; HIF1AN; HIF1N; Hypoxia inducible factor 1 alpha inhibitor; Hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia inducible factor asparagine hydroxylase; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide aspartate beta dioxygenase

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cancer; Epigenetics and Nuclear Signaling

Purification

Affinity purification

Concentration

0.6 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:300

Buffer

PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

40kDa

Isotype

IgG