P53RFP Polyclonal Antibody

Catalog number E-AB-15118-01

Choose a variant (4 options)

Selecting a variant updates its price, size and availability.

Price for E-AB-15118-01

Size / count 20 µL

Specifications

Catalog number: E-AB-15118-01
Size / count: 20 µL
Dry ice: No
Hazardous: No
Discontinued: No
Background: P53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.
Abbreviation: RNF144B
UniProt: Q7Z419
Host: Rabbit
Reactivity: Human; Mouse
Immunogen: Recombinant protein of human RNF144B
Target: BA528A10.3; E3 ubiquitin protein ligase RNF144B; E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain containing protein 2; IBR domain-containing protein 2; IBRDC 2; IBRDC2; KIAA0161; MGC71786; OTTHUMP00000016079; OTTHUMP00000016080; OTTHUMP00000039360; p53 inducible RING finger protein; p53-inducible RING finger protein; p53RFP; PIR2; R144B; Ring finger 144B; RING finger protein 144B; RNF 144B; RNF144B
Clonality: Polyclonal
Conjugation: Unconjugated
Applications: WB; IHC
Field of Research: Cell Biology
Purification: Affinity purification
Concentration: 0.4 mg/mL
Dilution: WB 1:200-1:1000; IHC 1:50-1:200
Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Shipping Conditions: The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight: 34kDa
Isotype: IgG

Frequently Asked Questions

What is the catalog number for P53RFP Polyclonal Antibody?

The catalog number for P53RFP Polyclonal Antibody is E-AB-15118.

What sizes are available for P53RFP Polyclonal Antibody?

P53RFP Polyclonal Antibody is available in the following size(s): 20 µL, 60 µL, 120 µL, 200 µL.

How many pack sizes does P53RFP Polyclonal Antibody come in?

P53RFP Polyclonal Antibody is available in 4 different pack sizes.

Is P53RFP Polyclonal Antibody in stock?

P53RFP Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures P53RFP Polyclonal Antibody?

P53RFP Polyclonal Antibody is manufactured by Elabscience.

What is the estimated delivery time for P53RFP Polyclonal Antibody?

Estimated delivery time for P53RFP Polyclonal Antibody is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for P53RFP Polyclonal Antibody?

The safety data sheet for P53RFP Polyclonal Antibody is available here: https://789.bio/eb/HGKOq1.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.