HLA-DOB Polyclonal Antibody

Specifications

Catalog number

E-AB-18081-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. HLA-DOB (Major Histocompatibility Complex, Class II, DO Beta) is a Protein Coding gene. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and CTLA4 Signaling. GO annotations related to this gene include MHC class II protein complex binding and MHC class II receptor activity. An important paralog of this gene is HLA-DRB1.

Abbreviation

HLA-DOB

UniProt

P13765

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide of human HLA-DOB

Target

HLA-DOB; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Immunology

Purification

Antigen affinity purification

Concentration

1.2 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:30-1:150

Buffer

PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

31 kDa

Observed Molecular Weight

Refer to figures

Isotype

IgG