HLA-DRB4 Polyclonal Antibody

Specifications

Catalog number

E-AB-18812-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Abbreviation

HLA-DRB4

UniProt

P13762

Host

Rabbit

Reactivity

Human

Immunogen

Fusion protein of human HLA-DRB4

Target

DR 4; DR beta 4 chain; DR4; DRB1 transplantation antigen ; DRB4; DRB4; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR beta 4 chain ; HLA-DRB4; Human leucocyte antigen DRB4 ; Leukocyte antigen ; Major histocompatibility complex class II DR beta 4 ; MHC class II antigen DRB4; MHC class II antigen HLA DR beta ; MHC class2 antigen ; MHC HLA DR-beta chain

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Immunology

Purification

Antigen affinity purification

Concentration

1.02 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:20-1:100

Buffer

PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

30 kDa

Observed Molecular Weight

Refer to figures

Isotype

IgG