DHX58 Polyclonal Antibody

Specifications

Catalog number

E-AB-19088-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3) . Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.

Abbreviation

DHX58

UniProt

Q96C10

Host

Rabbit

Reactivity

Human; Rat

Immunogen

Fusion protein of human DHX58

Target

D11LGP2; D11lgp2e; DEXH (Asp Glu X His) box polypeptide 58; DEXH box polypeptide 58; DHX 58; DHX58; DHX58; LGP 2; LGP2; Ortholog of mouse D11lgp2; Probable ATP dependent helicase LGP2; Probable ATP dependent RNA helicase DHX58; Probable ATP-dependent helicase LGP2; Probable ATP-dependent RNA helicase DHX58; Protein D11Lgp2 homolog; RIG-I-like receptor LGP2; RLR; RNA helicase LGP2

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cancer; Epigenetics and Nuclear Signaling; Immunology

Purification

Antigen affinity purification

Concentration

1.02 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:100

Buffer

PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

77 kDa

Observed Molecular Weight

Refer to figures

Isotype

IgG