CUL4A Polyclonal Antibody

Specifications

Catalog number

E-AB-19279-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B (104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Abbreviation

CUL4A

UniProt

Q13619

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Synthetic peptide of human CUL4A

Target

2810470J21Rik; AW495282; CUL 4A; CUL-4A; Cul4a; Cul4a protein; CUL4A; Cullin-4A; MGC36573; MGC64071

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cancer; Cell Biology

Purification

Antigen affinity purification

Concentration

1.62 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:300

Buffer

PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

88 kDa

Observed Molecular Weight

Refer to figures

Isotype

IgG