Phospho-Pan-Akt (Ser473) Polyclonal Antibody

Specifications

Catalog number

E-AB-20802-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity) . General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI (3) K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.

Abbreviation

Akt (phospho Ser473)

UniProt

P31749, P31751, Q9Y243

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human Akt around the phosphorylation site of Ser473

Target

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p; IF

Field of Research

Cancer; Neuroscience; Signal Transduction

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:50-1:200

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

56kDa

Observed Molecular Weight

55kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0304383521004031

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748231/

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.14275

Citation 04

https://journals.sagepub.com/doi/abs/10.1177/2515690X211006333

Isotype

IgG