Phospho-STAT1 (Tyr701) Polyclonal Antibody

Specifications

Catalog number

E-AB-20981-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs) . Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Abbreviation

Stat1 (phospho Tyr701)

UniProt

P42224

Host

Rabbit

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

Synthesized peptide derived from human Stat1 around the phosphorylation site of Tyr701

Target

Signal transducer and activator of transcription 1 91kD; CANDF7; DKFZp686B04100; IMD31A; IMD31B; IMD31C; ISGF 3; ISGF-3; OTTHUMP00000163552; OTTHUMP00000165046; OTTHUMP00000165047; OTTHUMP00000205845; Signal transducer and activator of transcription 1 91kDa; Signal transducer and activator of transcription 1; Signal transducer and activator of transcription 1;91kD; Signal transducer and activator of transcription 1-alpha/beta; STAT 1; Stat1; STAT1; STAT91; Transcription factor ISGF 3 components p91 p84; Transcription factor ISGF-3 components p91/p84

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p; IF

Field of Research

Cancer; Epigenetics and Nuclear Signaling; Signal Transduction

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:100-1:300

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

87kDa

Observed Molecular Weight

87kDa

Isotype

IgG