Histone H3 Monoclonal Antibody

Specifications

Catalog number

E-AB-22003-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Abbreviation

Histone H3

UniProt

P68431, Q71DI3, P84243

Host

Mouse

Reactivity

Human; Mouse; Rat; Yeast

Immunogen

Recombinant Protein

Target

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/

Clonality

Monoclonal

Clone

Clone:5B1

Conjugation

Unconjugated

Applications

WB; IF

Field of Research

Cancer; Epigenetics and Nuclear Signaling

Purification

Protein A purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-300; IF 1:100-500; IP 1:100-1:300

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

15kDa

Observed Molecular Weight

15kDa

Isotype

IgG