HIF1 bata Monoclonal Antibody

Specifications

Catalog number

E-AB-22189-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1) . The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2) . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4) . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7) .HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8) . Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8) .

Abbreviation

HIF-1 β/ARNT

UniProt

P27540

Host

Mouse

Reactivity

Mouse

Immunogen

Recombinant Protein

Target

ArnT; ARNT protein; ARNT; Aryl hydrocarbon receptor nuclear translocator; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF-1-beta; HIF1-beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Hypoxia-inducible factor 1-beta; nuclear translocator; Tango

Clonality

Monoclonal

Clone

4C5

Conjugation

Unconjugated

Applications

WB; IHC-p; IF

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism

Purification

Protein A purification

Concentration

1 mg/mL

Dilution

WB 1:1000-2000; IHC 1:100-200

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Observed Molecular Weight

87kDa

Isotype

IgG