Cleaved-CASP8 (D384) Polyclonal Antibody

Specifications

Catalog number

E-AB-30009-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Abbreviation

Cleaved-Caspase-8 (D384)

UniProt

Q14790

Host

Rabbit

Reactivity

Human

Immunogen

Synthesized peptide derived from the C-terminal region of human Caspase-8

Target

ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptotic cysteine protease; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP4; CASP-8; CASP8; CASP8; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase-8 subunit p10; CED 3; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MCH5; MGC78473; MORT1 associated ced 3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p; IF

Field of Research

Cancer; Cell Biology; Metabolism

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-2000; IHC 1:50-300; IF 1:50-300

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

55kDa

Observed Molecular Weight

47+55kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332220302456

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.22943

Isotype

IgG